Knocking out OsNAC050 Expression Causes Low-Temperature Tolerance in Rice by Regulating Photosynthesis and the Sucrose Metabolic Pathway

Because rice is native to tropical and subtropical regions, it generally more sensitive cold stress compared other cereals. Low-temperature has a strong influence on the growth, development, yield of rice. Plant NAM, ATAF1/2 CUC(NAC) transcription factors (TFs) play significant regulatory roles in response. In our research, we found that OsNAC050 (LOC_Os03g60080) mainly expressed leaves. Cold (4 °C), heat (42 PEG 6000 (20%, w/v), NaCl (200 mM), H2O2 (1%), IAA (100 μM), ABA μM) GA3 were used treat wild type (WT) plants. A low temperature further up-regulated expression. mutants created using CRISPR-Cas9 gene editing technology showed significantly enhanced tolerance low-temperature treatment. The measurement enzyme activities related redox pathway also have stronger viability under stress. Comparative transcriptome analysis photosynthesis soluble sugar metabolism affected mutant lines, suggesting may participate above molecular pathways response results expand understanding mechanisms underlying responses can provide new strategies for engineering high-yielding varieties.